Biotechnology

PROGRAMME

Monday, August 26th

13.00-18.00	Arrival and accommodation of the course participants
18.00-19.00	Opening introduction to the course Estonian Biocentre 23 Riia St. room 218 Tel: +372 7 375 029; Fax: +372 7 420 286 http://www.biotech.ebc.ee http://www.ut.ee
18.00-18.30	Prof. Andres Metspalu Chairman of the Course
18.30-19.00	A tour in the Institute of Molecular and Cell Biology and Estonian Biocentre, 23 Riia St. 51010 Tartu
20.00	Dinner and "get together" party at Tartu City Museum, Narva Road 23

Tuesday, August 27th

9.00-12.00	Silanization of the slides	46 Vanemuise St.
9.00-9.30	Lecture "Introduction to slide chemistry", Ants Kurg	Room 301
9.30-12.00	Practical work: Slide silanization technology	Room 209
12.30-13.30	Lunch at "Kotka Kelder" 14 Pepleri St
13.30-18.00	PCR of templates	46 Vanemuise St
Experiment 1.	Detection ß-thalassemia mutation by APEX	Room 209
Experiment 2	SNP genotyping by APEX	Room 209
Experiment 3.	Resequencing of p53 gene by APEX	Room 209
15.00-17.00	Discussion. Participants will be asked to introduce shortly their own research projects	Room 301
17.15-18.00	Practical work continues: Control of the PCR products by agarose gel electrophoresis. Ethanol precipitation of PCR products.	Room 209
18.00	Discussion of the day	Room 301

Wednesday, August 28th

9.00-9.45	Practical work: Slide silanization continues	46 Vanemuise St. Room 209
9.45-11.30	Spotting of the slides with Affymetrix-417 spotter Bus transfer to "Asper Biotech" facility 5 min	3 Oru St.
11.30-12.30	Practical work: Slide silanization will be finished	46 Vanemuise St. Room 209
12.30-13.30	Lunch at "Kotka Kelder" 14 Pepleri St.
13.30	Group photo (at Riia 23 main door)
13.35-16.00	Preparation of template	46 Vanemuise St.
13.30-16.00	Practical work: Fragmentation of PCR products by sAP and UNG treatment. Control of fragmentation	Room 209
16.00-18.00	Discussion. Participants will be asked to introduce shortly their own research projects	Room 301
18.00	Discussion of the day	Room 301

Thursday, August 29th

9.00-12.00	Arrayed primer extension experiments	46 Vanemuise St.
9.00-9.30	Lecture "Imaging of APEX slides by TIRF detector", Ants Kurg	Room 301
9.30-11.30	Practical work: Quality control of spotted slides by SYBRGreen II staining followed by imaging with Genorama-003 detector	Room 209
11.30-12.00	Lecture " Introduction to APEX image analysis" Neeme Tõnisson	Room 301
12.30-13.30	Lunch at "Kotka Kelder" 14 Pepleri St.
13.30-18.00	APEX experiments	Room 209
Experiment 1.	Detection ß-thalassemia mutation by APEX	Room 209
Experiment 2.	SNP genotyping by APEX	Room 209
Experiment 3.	Resequencing of p53 gene by APEX	Room 209
17.00-17.30	Introduction lecture: "How to use Genorama software for different applications", Neeme Tõnisson	Room 301
17.30-18.00	Practical work: Imaging of APEX slides using Genorama-003 detector	Room 209
18.00	Discussion of the day	Room 301

Friday, August 30th

8.30-12.00	Analysis of arrays, final discussion and conclusions	46 Vanemuise St.
8.30-10.00	Practical work: Analysis of APEX results First Group	Room 107
10.00-11.30	Practical work: Analysis of APEX results Second Group	Room 107
11.30-12.00	Conclusion: Prof. Andres Metspalu	Room 301
12.00	Lunch
13.00-19.00	Excursion to Lahemaa National Park in Northern Estonia.
19.00	Dinner at Altja Tavern and transfer to Tartu.
22.30	Arrival in Tartu.

August 31. - September 1.

Lecture course "Theory and Different Practical Methods of Genotyping"
Venue: Hotel London conference hall, Tartu.

Saturday, August 31st

10.00-10.10	Prof. Andres Metspalu: Introduction to the course
11.00-11.50	Prof. Anne-Christine Syv�nen, Uppsala University, Sweden: "Multiplex, quantitative genotyping of SNPs by minisequencing using microarrays"
11.50-12.20	Coffee/tea break
12.20-13.10	Dr. Markus Beier, Director Application, Febit AG,Germany: "Exploring natures genetic diversity with a flexibleprobing tool"
13.10-14.00	Prof. Anthony Brookes, Centre for Genomics and Bioinformatics, Karolinska Institutet, Sweden: "Ultra-High throughput SNP genotyping upon centrifugal arrays by dynamic allele specific hybradization (DASH)"
14.00-15.10	Lunch at the hotel London
15.10-16.00	Dr. Scott White, Center for Human Genome Studies, Bioscience Division, Los Alamos, USA: "High-throughput SNP scoring with GAMMArrays: genomic analysis using Multiplexed Microsphere Arrays"
16.00-16.50	Dr. Niels Storm, Sr. Scientist, Sequenom GmbH, Germany: "SNP Discovery and SNP Scoring with MALDI-TOF Mass Spectrometry"
16.50-17.10	Coffee/tea break
17.10-18.00	Dr. Per Johan Ulfendahl, Director, Intellectual Properties, Pyrosequencing AB, Sweden: "PyrosequencingTM is sequencing by synthesis, a simple to use technique for accurate and consistent analysis of DNA sequences"
18.00-18.30	Discussion
20.00	Buffet Dinner at restaurant Atlantis (located at Narva Road 2)

Sunday, September 1st

10.00-10.50	Prof. Andres Metspalu, University of Tartu/Estonian Biocentre, Estonia: "Large scale SNP typing: chr22 as a model"
10.50-11.35	Dr. Maido Remm, Estonian Biocentre, Estonia: "Software tools for selection of SNP-s and primer design"
11.35-11.45	Prof. Andres Metspalu: Closing remarks
12.00-13.00	Lunch at the hotel London
13.20-14.00	Departure of the course participants to the Tallinn Airport.